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KMID : 0900919950190010049
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Korean journal of Animal Reproduction
1995 Volume.19 No. 1 p.49 ~ p.54
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Pregnancy and Survival Rate of In Vitro Fertilized Bovine Embryos Frozen for Direct Transfer
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Oh S.-J.
Yang B.-S.
Lee Myung -Shik
Baek Kwang-Soo
Seong Hwan-Hoo
Lim Kyung-Sun
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Abstract
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This experiments were carried out to investigate the viabilities and the pregnancy rate of frozen-thawed IVF bovine embryos in various media, cryoprotectants and age of embryos produced. Hanwoo oocyte were collected in size of 2~7mm follicles, matured for 20~22hrs at 38.5^{circ}C in 5% CO2 incubator and then in vitro fertilized with Hanwoo semen. Blastocysts or more developed embryos at Day 7, 8 and 9 were frozen in 1.5 or 1.8M ethylene glycol. Viability of frozen thawed IVF embryos were identified the reformation of blastocoele after thawing and culture for 24~48 hours at 38.5^{circ}C in 5% CO2 incubator. Production rate of Hanwoo IVF embryos in TCM 199 and CR1aa ws 21.3%(39/183) and 28.1%(41/146), respectively. The viability of frozen thawed IVF embryos was higher rate in 1.8M ethylene glycol and Day 7 embryos than that in 1.5M and Day 8.53 cows out of 100 Hanwoo receipients transfered IVF embryos were pregnant and twin production rate was 26.3%.
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KEYWORD
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IVF embryos, Hanwoo, direct transfer, frozen-thawed embryos, viability
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